6a7e

X-ray diffraction
1.85Å resolution

Human dihydrofolate reductase complexed with NADPH and BT2

Released:

Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00374 (Residues: 2-187; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: R3
Unit cell:
a: 84.755Å b: 84.755Å c: 77.726Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.238
Expression system: Escherichia coli BL21(DE3)