› 6a71

X-ray diffraction
1.6Å resolution

Crystal Structure of Human ATP7B and TM Complex

Released:
DOI: 10.2210/pdb6a71/pdb
PDB entry 6a71 coloured by chain, front view.
PDB entry 6a71 coloured by chain, side view.
PDB entry 6a71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tetrathiomolybdate induces dimerization of the metal-binding domain of ATPase and inhibits platination of the protein.
Fang T, Chen W, Sheng Y, Yuan S, Tang Q, Li G, Huang G, Su J, Zhang X, Zang J, Liu Y
Nat Commun 10 186 (2019)
PMID: 30643139

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Cu(+)(Side 1) = ADP + phosphate + Cu(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-transporting ATPase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 7.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35670 (Residues: 357-428; Coverage: 5%)
Gene names: ATP7B, PWD, WC1, WND
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

4 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
Ligand CA 1 x CA
Ligand 9UX 1 x 9UX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 46.267Å b: 87.74Å c: 78.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.195
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Drug-Resistance Mechanisms of Five Platinum-Based Antitumor Agents.
Zhou et al. (2020)
5 more

1 mention without citation

Structure of the Wilson disease copper transporter ATP7B.
Bitter et al. (2022)