› 6a6n

X-ray diffraction
3.02Å resolution

Crystal structure of an inward-open apo state of the eukaryotic ABC multidrug transporter CmABCB1

Released:
DOI: 10.2210/pdb6a6n/pdb
PDB entry 6a6n coloured by chain, front view.
PDB entry 6a6n coloured by chain, side view.
PDB entry 6a6n coloured by chain, top view.
Source organism: Cyanidioschyzon merolae strain 10D
Primary publication:
Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1.
Kodan A, Yamaguchi T, Nakatsu T, Matsuoka K, Kimura Y, Ueda K, Kato H
OpenAccess logo Nat Commun 10 88 (2019)
PMID: 30622258

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent transporter ycf16 Chain: A
Molecule details ›
Chain: A
Length: 612 amino acids
Theoretical weight: 66.05 KDa
Source organism: Cyanidioschyzon merolae strain 10D
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: M1VAN7 (Residues: 93-696; Coverage: 87%)
Gene name: CYME_CMD148C
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand DMU 4 x DMU
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R32
Unit cell:
a: 179.69Å b: 179.69Å c: 157.666Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.275
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 review citations

Mechanics and pharmacology of substrate selection and transport by eukaryotic ABC exporters.
Srikant et al. (2019)
8 more

3 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
2 more