› 6a5f

X-ray diffraction
2.05Å resolution

The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of nargenicin

Released:
DOI: 10.2210/pdb6a5f/pdb
PDB entry 6a5f coloured by chain, front view.
PDB entry 6a5f coloured by chain, side view.
PDB entry 6a5f coloured by chain, top view.
Source organism: Nocardia argentinensis ATCC 31306
Primary publication:
Enzyme-catalysed [6+4] cycloadditions in the biosynthesis of natural products.
Zhang B, Wang KB, Wang W, Wang X, Liu F, Zhu J, Shi J, Li LY, Han H, Xu K, Qiao HY, Zhang X, Jiao RH, Houk KN, Liang Y, Tan RX, Ge HM
Nature 568 122-126 (2019)
PMID: 30867595

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NgnD Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 18.09 KDa
Source organism: Nocardia argentinensis ATCC 31306
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 116.99Å b: 116.99Å c: 63.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.184
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Discovery and investigation of natural Diels-Alderases.
Watanabe K. (2021)
19 other articles

1 mention without citation

Enzyme-catalysed [6+4] cycloadditions in the biosynthesis of natural products.
Zhang et al. (2019)