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› 6a5e

X-ray diffraction
2.77Å resolution

Crystal structure of plant peptide RALF23 in complex with FER and LLG2

Released:
DOI: 10.2210/pdb6a5e/pdb
PDB entry 6a5e coloured by chain, front view.
PDB entry 6a5e coloured by chain, side view.
PDB entry 6a5e coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of RALF peptide perception by a heterotypic receptor complex.
Xiao Y, Stegmann M, Han Z, DeFalco TA, Parys K, Xu L, Belkhadir Y, Zipfel C, Chai J
Nature 572 270-274 (2019)
PMID: 31291642

Function and Biology Details

Reaction catalysed: 
[(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)., (2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP +H(+)., (1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)., (2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP +H(+).]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-180060 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Receptor-like protein kinase FERONIA Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9SCZ4 (Residues: 28-423; Coverage: 46%)
Gene names: AAK1, At3g51550, F26O13.190, FER, SIR, SRN
Sequence domains: Malectin-like domain
GPI-anchored protein LLG2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 84 amino acids
Theoretical weight: 9.37 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q6NLF4 (Residues: 42-125; Coverage: 60%)
Gene names: At2g20700, LLG2
Rapid alkalinization factor 23 Chains: E, F
Molecule details ›
Chains: E, F
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Arabidopsis
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9LUS7 (Residues: 92-106; Coverage: 14%)
Gene names: At3g16570, MGL6.2, RALF23, RALFL23

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 62.312Å b: 62.36Å c: 97.478Å
α: 105.72° β: 91.3° γ: 108.48°
R-values:
R R work R free
0.235 0.232 0.285
Expression system: Insect cell expression vector pTIE1

Quick links

Citations

38 review citations

Thirty years of resistance: Zig-zag through the plant immune system.
Ngou et al. (2022)
37 more

3 mentions without citation

Malectin-like receptor kinases as protector deities in plant immunity.
Ortiz-Morea et al. (2022)
2 more