› 6a2f

X-ray diffraction
2.5Å resolution

Crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6a2f/pdb
PDB entry 6a2f coloured by chain, front view.
PDB entry 6a2f coloured by chain, side view.
PDB entry 6a2f coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
Dong H, Han Q, Guo Y, Ju J, Wang S, Yuan C, Long W, He X, Xu S, Li S
Biochem Biophys Res Commun 503 2319-2325 (2018)
PMID: 29964014

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase, biosynthetic Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 38.58 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUN4 (Residues: 1-358; Coverage: 100%)
Gene names: PA4930, alr
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MLI 2 x MLI
Ligand DLY 2 x DLY
Ligand ACT 2 x ACT
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 74.24Å b: 78.3Å c: 154.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.238
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structure of the d-Cycloserine-Resistant Variant D322N of Alanine Racemase from Mycobacterium tuberculosis.
de Chiara et al. (2023)