X-ray diffraction
1.39Å resolution

The crystal structure of Mandelate oxidase with 3-fluoropyruvate


Function and Biology Details

Reaction catalysed:
(S)-4-hydroxymandelate + O(2) = 2-(4-hydroxyphenyl)-2-oxoacetate + H(2)O(2) 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
4-hydroxymandelate oxidase Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 40.05 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
  • Canonical: O52792 (Residues: 1-357; Coverage: 100%)
Gene name: hmo
Sequence domains: FMN-dependent dehydrogenase

Ligands and Environments

Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: I422
Unit cell:
a: 138.096Å b: 138.096Å c: 111.203Å
α: 90° β: 90° γ: 90°
R R work R free
0.179 0.179 0.193
Expression system: Escherichia coli