6a01

X-ray diffraction
1.87Å resolution

The crystal structure of Mandelate oxidase Y128F with 3,3-difluoro-2,2-dihydroxy-3-phenylpropionic acid

Released:
Source organism: Amycolatopsis orientalis
Entry authors: Li TL, Lin KH

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxymandelate oxidase Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 40.03 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52792 (Residues: 1-357; Coverage: 100%)
Gene name: hmo
Sequence domains: FMN-dependent dehydrogenase

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: I422
Unit cell:
a: 138.204Å b: 138.204Å c: 111.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.2
Expression system: Escherichia coli