6yih

X-ray diffraction
2.55Å resolution

Structure of Chromosomal Passenger Complex (CPC) bound to phosphorylated Histone 3 peptide at 2.6 A.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Baculoviral IAP repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15392 (Residues: 1-142; Coverage: 100%)
Gene names: API4, BIRC5, IAP4
Sequence domains: Inhibitor of Apoptosis domain
Borealin Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 8.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53HL2 (Residues: 10-76; Coverage: 24%)
Gene names: CDCA8, PESCRG3
Sequence domains: Nbl1 / Borealin N terminal
Inner centromere protein Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 9.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NQS7 (Residues: 5-80; Coverage: 8%)
Gene name: INCENP
Sequence domains: Chromosome passenger complex (CPC) protein INCENP N terminal
Histone H3.1 Chain: D
Molecule details ›
Chain: D
Length: 12 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-13; Coverage: 9%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P65
Unit cell:
a: 99.365Å b: 99.365Å c: 56.224Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.234 0.259
Expression systems:
  • Escherichia coli
  • Not provided