6yie

X-ray diffraction
3.49Å resolution

Structure of a Borealin-INCENP-Survivin complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Baculoviral IAP repeat-containing protein 5 Chains: A, D
Molecule details ›
Chains: A, D
Length: 144 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15392 (Residues: 1-142; Coverage: 100%)
Gene names: API4, BIRC5, IAP4
Sequence domains: Inhibitor of Apoptosis domain
Borealin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53HL2 (Residues: 10-109; Coverage: 36%)
Gene names: CDCA8, PESCRG3
Sequence domains: Nbl1 / Borealin N terminal
Inner centromere protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 60 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NQS7 (Residues: 1-58; Coverage: 6%)
Gene name: INCENP
Sequence domains: Chromosome passenger complex (CPC) protein INCENP N terminal

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P212121
Unit cell:
a: 59.536Å b: 78.824Å c: 125.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.269 0.324
Expression system: Escherichia coli BL21(DE3)