› 6x8a

X-ray diffraction
1.06Å resolution

Sucrose-bound structure of Marinomonas primoryensis PA14 carbohydrate-binding domain

Released:
DOI: 10.2210/pdb6x8a/pdb
PDB entry 6x8a coloured by chain, front view.
PDB entry 6x8a coloured by chain, side view.
PDB entry 6x8a coloured by chain, top view.
Source organism: Marinomonas primoryensis
Primary publication:
Structural Basis of Ligand Selectivity by a Bacterial Adhesin Lectin Involved in Multispecies Biofilm Formation.
Guo S, Vance TDR, Zahiri H, Eves R, Stevens C, Hehemann JH, Vidal-Melgosa S, Davies PL
mBio 12 (2021)
PMID: 33824212

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cadherin-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 20.18 KDa
Source organism: Marinomonas primoryensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A1YIY3 (Residues: 716-904; Coverage: 12%)

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 45.23Å b: 50.61Å c: 79.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.162
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Formation, Development, and Cross-Species Interactions in Biofilms.
Luo et al. (2021)
1 more

1 mention without citation

Structural Basis of Ligand Selectivity by a Bacterial Adhesin Lectin Involved in Multispecies Biofilm Formation.
Guo et al. (2021)