X-ray diffraction
1.4Å resolution

Rev1 Ternary Complex with dCTP and Ca2+

Primary publication:
Visualizing Rev1 catalyze protein-template DNA synthesis.
Proc Natl Acad Sci U S A 117 25494-25504 (2020)
PMID: 32999062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 442 amino acids
Theoretical weight: 50.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P12689 (Residues: 305-746; Coverage: 45%)
Gene names: O6339, REV1, YOR346W
Sequence domains: impB/mucB/samB family
DNA (5'-D(*GP*GP*GP*GP*TP*GP*TP*GP*GP*TP*AP*G)-3') Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.81 KDa
Source organism: synthetic construct
Expression system: Not provided
Molecule details ›
Chain: T
Length: 17 nucleotides
Theoretical weight: 5.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 62.376Å b: 73.947Å c: 117.29Å
α: 90° β: 90° γ: 90°
R R work R free
0.187 0.186 0.207
Expression systems:
  • Escherichia coli
  • Not provided