6way

X-ray diffraction
1.5Å resolution

C-terminal SH2 domain of p120RasGAP in complex with p190RhoGAP phosphotyrosine peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras GTPase-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20936 (Residues: 340-444; Coverage: 10%)
Gene names: GAP, RASA, RASA1
Sequence domains: SH2 domain
Rho GTPase-activating protein 35 Chain: V
Molecule details ›
Chain: V
Length: 10 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NRY4 (Residues: 1086-1093; Coverage: 1%)
Gene names: ARHGAP35, GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 63.119Å b: 83.91Å c: 54.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.19
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided