6w6l

Electron Microscopy
3.84Å resolution

Cryo-EM structure of the human ribosome-TMCO1 translocon

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
Entry contents:
49 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (54 distinct):
60S ribosomal protein L8 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62917 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Sequence domains:
60S ribosomal protein L3 Chain: B
Molecule details ›
Chain: B
Length: 403 amino acids
Theoretical weight: 46.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39023 (Residues: 1-403; Coverage: 100%)
Gene names: OK/SW-cl.32, RPL3
Sequence domains: Ribosomal protein L3
60S ribosomal protein L4 Chain: C
Molecule details ›
Chain: C
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P36578 (Residues: 1-427; Coverage: 100%)
Gene names: RPL1, RPL4
Sequence domains:
60S ribosomal protein L5 Chain: F
Molecule details ›
Chain: F
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46777 (Residues: 1-297; Coverage: 100%)
Gene names: MSTP030, RPL5
Sequence domains:
60S ribosomal protein L6 Chain: G
Molecule details ›
Chain: G
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02878 (Residues: 1-288; Coverage: 100%)
Gene names: RPL6, TXREB1
Sequence domains:
60S ribosomal protein L7 Chain: H
Molecule details ›
Chain: H
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18124 (Residues: 1-248; Coverage: 100%)
Gene name: RPL7
Sequence domains:
60S ribosomal protein L7a Chain: I
Molecule details ›
Chain: I
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62424 (Residues: 1-266; Coverage: 100%)
Gene names: RPL7A, SURF-3, SURF3
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L9 Chain: J
Molecule details ›
Chain: J
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32969 (Residues: 1-192; Coverage: 100%)
Gene names: OK/SW-cl.103, RPL9, RPL9P7, RPL9P8, RPL9P9
Sequence domains: Ribosomal protein L6
60S ribosomal protein L10 Chain: K
Molecule details ›
Chain: K
Length: 214 amino acids
Theoretical weight: 24.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P27635 (Residues: 1-214; Coverage: 100%)
Gene names: DXS648E, QM, RPL10
Sequence domains: Ribosomal protein L16p/L10e
60S ribosomal protein L11 Chain: L
Molecule details ›
Chain: L
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62913 (Residues: 1-178; Coverage: 100%)
Gene name: RPL11
Sequence domains:
60S ribosomal protein L13 Chain: M
Molecule details ›
Chain: M
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Sequence domains: Ribosomal protein L13e
60S ribosomal protein L14 Chain: N
Molecule details ›
Chain: N
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50914 (Residues: 1-215; Coverage: 100%)
Gene name: RPL14
Sequence domains: Ribosomal protein L14
60S ribosomal protein L15 Chain: O
Molecule details ›
Chain: O
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61313 (Residues: 1-204; Coverage: 100%)
Gene names: EC45, RPL15, TCBAP0781
Sequence domains: Ribosomal L15
60S ribosomal protein L13a Chain: P
Molecule details ›
Chain: P
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40429 (Residues: 1-203; Coverage: 100%)
Gene name: RPL13A
Sequence domains: Ribosomal protein L13
60S ribosomal protein L17 Chain: Q
Molecule details ›
Chain: Q
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Sequence domains: Ribosomal protein L22p/L17e
60S ribosomal protein L18 Chain: R
Molecule details ›
Chain: R
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07020 (Residues: 1-188; Coverage: 100%)
Gene name: RPL18
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
60S ribosomal protein L19 Chain: S
Molecule details ›
Chain: S
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P84098 (Residues: 1-196; Coverage: 100%)
Gene name: RPL19
Sequence domains: Ribosomal protein L19e
60S ribosomal protein L18a Chain: T
Molecule details ›
Chain: T
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02543 (Residues: 1-176; Coverage: 100%)
Gene name: RPL18A
Sequence domains: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
60S ribosomal protein L21 Chain: U
Molecule details ›
Chain: U
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46778 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
60S ribosomal protein L22 Chain: V
Molecule details ›
Chain: V
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35268 (Residues: 1-128; Coverage: 100%)
Gene name: RPL22
Sequence domains: Ribosomal L22e protein family
60S ribosomal protein L23 Chain: W
Molecule details ›
Chain: W
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
60S ribosomal protein L24 Chain: X
Molecule details ›
Chain: X
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P83731 (Residues: 1-157; Coverage: 100%)
Gene name: RPL24
Sequence domains: Ribosomal protein L24e
60S ribosomal protein L23a Chain: Y
Molecule details ›
Chain: Y
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62750 (Residues: 1-156; Coverage: 100%)
Gene name: RPL23A
Sequence domains:
60S ribosomal protein L26 Chain: Z
Molecule details ›
Chain: Z
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61254 (Residues: 1-145; Coverage: 100%)
Gene name: RPL26
Sequence domains:
60S ribosomal protein L27 Chain: a
Molecule details ›
Chain: a
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61353 (Residues: 1-136; Coverage: 100%)
Gene name: RPL27
Sequence domains:
60S ribosomal protein L27a Chain: b
Molecule details ›
Chain: b
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46776 (Residues: 1-148; Coverage: 100%)
Gene name: RPL27A
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
60S ribosomal protein L29 Chain: c
Molecule details ›
Chain: c
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47914 (Residues: 1-159; Coverage: 100%)
Gene name: RPL29
Sequence domains: Ribosomal L29e protein family
60S ribosomal protein L30 Chain: d
Molecule details ›
Chain: d
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62888 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L31 Chain: e
Molecule details ›
Chain: e
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
60S ribosomal protein L32 Chain: f
Molecule details ›
Chain: f
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62910 (Residues: 1-135; Coverage: 100%)
Gene names: PP9932, RPL32
Sequence domains: Ribosomal protein L32
60S ribosomal protein L35a Chain: g
Molecule details ›
Chain: g
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18077 (Residues: 1-110; Coverage: 100%)
Gene names: GIG33, RPL35A
Sequence domains: Ribosomal protein L35Ae
60S ribosomal protein L34 Chain: h
Molecule details ›
Chain: h
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49207 (Residues: 1-117; Coverage: 100%)
Gene name: RPL34
Sequence domains: Ribosomal protein L34e
60S ribosomal protein L35 Chain: i
Molecule details ›
Chain: i
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42766 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
60S ribosomal protein L36 Chain: j
Molecule details ›
Chain: j
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y3U8 (Residues: 1-105; Coverage: 100%)
Gene name: RPL36
Sequence domains: Ribosomal protein L36e
60S ribosomal protein L37 Chain: k
Molecule details ›
Chain: k
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61927 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Sequence domains: Ribosomal protein L37e
60S ribosomal protein L38 Chain: l
Molecule details ›
Chain: l
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63173 (Residues: 1-70; Coverage: 100%)
Gene name: RPL38
Sequence domains: Ribosomal L38e protein family
60S ribosomal protein L39 Chain: m
Molecule details ›
Chain: m
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62891 (Residues: 1-51; Coverage: 100%)
Gene name: RPL39
Sequence domains: Ribosomal L39 protein
60S ribosomal protein L41 Chain: o
Molecule details ›
Chain: o
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62945 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Sequence domains: Ribosomal protein L41
60S ribosomal protein L36a Chain: p
Molecule details ›
Chain: p
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: J3KQN4 (Residues: 37-142; Coverage: 75%)
Gene name: RPL36A
Sequence domains: Ribosomal protein L44
60S ribosomal protein L37a Chain: q
Molecule details ›
Chain: q
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61513 (Residues: 1-92; Coverage: 100%)
Gene name: RPL37A
Sequence domains: Ribosomal L37ae protein family
60S ribosomal protein L28 Chain: r
Molecule details ›
Chain: r
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46779 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Sequence domains: Ribosomal L28e protein family
Nascent chain mixture Chain: y
Molecule details ›
Chain: y
Length: 27 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Protein transport protein Sec61 subunit alpha isoform 1 Chain: 1
Molecule details ›
Chain: 1
Length: 476 amino acids
Theoretical weight: 52.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61619 (Residues: 1-476; Coverage: 100%)
Gene names: SEC61A, SEC61A1
Sequence domains:
Protein transport protein Sec61 subunit gamma Chain: 2
Molecule details ›
Chain: 2
Length: 68 amino acids
Theoretical weight: 7.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60059 (Residues: 1-68; Coverage: 100%)
Gene name: SEC61G
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein transport protein Sec61 subunit beta Chain: 3
Molecule details ›
Chain: 3
Length: 96 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60468 (Residues: 1-96; Coverage: 100%)
Gene name: SEC61B
Sequence domains: Sec61beta family
Transmembrane protein 147 Chain: 4
Molecule details ›
Chain: 4
Length: 224 amino acids
Theoretical weight: 25.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BVK8 (Residues: 1-224; Coverage: 100%)
Gene name: TMEM147
Sequence domains: Predicted membrane protein (DUF2053)
Nicalin Chain: 5
Molecule details ›
Chain: 5
Length: 563 amino acids
Theoretical weight: 63.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q969V3 (Residues: 1-563; Coverage: 100%)
Gene name: NCLN
Sequence domains: Peptidase family M28
PAT complex subunit CCDC47 Chain: 7
Molecule details ›
Chain: 7
Length: 483 amino acids
Theoretical weight: 55.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96A33 (Residues: 1-483; Coverage: 100%)
Gene names: CCDC47, GK001, MSTP041, PSEC0077
Sequence domains: PAT complex subunit CCDC47
Calcium load-activated calcium channel Chain: 6
Molecule details ›
Chain: 6
Length: 188 amino acids
Theoretical weight: 21.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UM00 (Residues: 52-239; Coverage: 79%)
Gene names: PNAS-10, PNAS-136, TMCC4, TMCO1, UNQ151/PRO177
5.8S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 157 nucleotides
Theoretical weight: 50.45 KDa
Sequence domains: 5.8S ribosomal RNA
5S ribosomal RNA Chain: E
Molecule details ›
Chain: E
Length: 119 nucleotides
Theoretical weight: 38.39 KDa
Sequence domains: 5S ribosomal RNA
28S ribosomal RNA Chain: t
Molecule details ›
Chain: t
Length: 3579 nucleotides
Theoretical weight: 1159.53 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
tRNA Chain: u
Molecule details ›
Chain: u
Length: 76 nucleotides
Theoretical weight: 24.41 KDa
Sequence domains: tRNA
tRNA Chain: v
Molecule details ›
Chain: v
Length: 76 nucleotides
Theoretical weight: 24.44 KDa
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.84Å
Relevant EMDB volumes: EMD-21435