› 6ths

X-ray diffraction
1.1Å resolution

High resolution crystal structure of Leaf-branch cutinase S165A variant

Released:
DOI: 10.2210/pdb6ths/pdb
PDB entry 6ths coloured by chain, front view.
PDB entry 6ths coloured by chain, side view.
PDB entry 6ths coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
An engineered PET depolymerase to break down and recycle plastic bottles.
Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guémard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, André I, Duquesne S, Marty A
Nature 580 216-219 (2020)
PMID: 32269349

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leaf-branch compost cutinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.78 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G9BY57 (Residues: 36-293; Coverage: 100%)
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

1 bound ligand:
Ligand DIO 1 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P63
Unit cell:
a: 109.604Å b: 109.604Å c: 35.287Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.136 0.167
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

65 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
64 more

7 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
6 more