6t0u

Electron Microscopy
3.12Å resolution

Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with intact oligo(U) sequence

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 738 amino acids
Theoretical weight: 85.43 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 809 amino acids
Theoretical weight: 91.03 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
vRNA Chain: V
Molecule details ›
Chain: V
Length: 44 nucleotides
Theoretical weight: 13.91 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-10359
Expression system: Trichoplusia ni