6t0n

Electron Microscopy
2.54Å resolution

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 738 amino acids
Theoretical weight: 85.49 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 809 amino acids
Theoretical weight: 91.03 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
5' vRNA Chain: V
Molecule details ›
Chain: V
Length: 14 nucleotides
Theoretical weight: 4.56 KDa
3' vRNA Chain: R
Molecule details ›
Chain: R
Length: 16 nucleotides
Theoretical weight: 4.95 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-10356
Expression system: Trichoplusia ni