› 6sqq

X-ray diffraction
2.37Å resolution

Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant in complex with RNA obtained by soaking

Released:
DOI: 10.2210/pdb6sqq/pdb
PDB entry 6sqq coloured by chain, front view.
PDB entry 6sqq coloured by chain, side view.
PDB entry 6sqq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expanding crystallization tools for nucleic acid complexes using U1A protein variants.
Rosenbach H, Victor J, Borggräfe J, Biehl R, Steger G, Etzkorn M, Span I
J Struct Biol 210 107480 (2020)
PMID: 32070773

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140523 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: AAA, BBB, CCC
Molecule details ›
Chains: AAA, BBB, CCC
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
RNA hairpin Chains: XXX, YYY, ZZZ
Molecule details ›
Chains: XXX, YYY, ZZZ
Length: 21 nucleotides
Theoretical weight: 6.61 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P6522
Unit cell:
a: 96.08Å b: 96.08Å c: 258.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.245 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.
Rosenbach et al. (2020)
1 more