› 6s99

X-ray diffraction
2.65Å resolution

Dimethylated fusion protein of RSL and trimeric coiled coil in complex with cucurbit[7]uril

Released:
DOI: 10.2210/pdb6s99/pdb
PDB entry 6s99 coloured by chain, front view.
PDB entry 6s99 coloured by chain, side view.
PDB entry 6s99 coloured by chain, top view.
Source organism: Ralstonia solanacearum
Primary publication:
Segregated Protein-Cucurbit[7]uril Crystalline Architectures via Modulatory Peptide Tectons.
Ramberg KO, Guagnini F, Engilberge S, Wrońska MA, Rennie ML, Pérez J, Crowley PB
Chemistry 27 14619-14627 (2021)
PMID: 34432924

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-binding lectin protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 114 amino acids
Theoretical weight: 12.37 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0S4TLR1 (Residues: 2-91; Coverage: 99%)
Gene names: E7Z57_08365, HF909_06975, LBW55_09125, RUN39_v1_50103
Sequence domains: Fungal fucose-specific lectin

Ligands and Environments

2 bound ligands:
Ligand BMA 12 x BMA
Ligand QQ7 6 x QQ7
1 modified residue:
Ligand MLY 42 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 51.219Å b: 51.242Å c: 111.282Å
α: 76.8° β: 89.88° γ: 60.05°
R-values:
R R work R free
0.226 0.224 0.256
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Understanding a protein fold: The physics, chemistry, and biology of α-helical coiled coils.
Woolfson DN. (2023)
1 other article

1 mention without citation

Segregated Protein-Cucurbit[7]uril Crystalline Architectures via Modulatory Peptide Tectons.
Ramberg et al. (2021)