› 6qpd

X-ray diffraction
2Å resolution

Engineered beta-lactoglobulin: variant I56F

Released:
DOI: 10.2210/pdb6qpd/pdb
PDB 6qpd coloured by chain and viewed from the front.
PDB 6qpd coloured by chain and viewed from the side.
PDB 6qpd coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Structure-based design approach to rational site-directed mutagenesis of β-lactoglobulin.
Bonarek P, Loch JI, Tworzydło M, Cooper DR, Milto K, Wróbel P, Kurpiewska K, Lewiński K
J Struct Biol 210 107493 (2020)
PMID: 32169624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.34 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P3221
Unit cell:
a: 53.272Å b: 53.272Å c: 113.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.207 0.269
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Milk Processing Affects Structure, Bioavailability and Immunogenicity of β-lactoglobulin.
Broersen K. (2020)