› 6qh6

X-ray diffraction
5Å resolution

AP2 clathrin adaptor core with two cargo peptides in open+ conformation

Released:
DOI: 10.2210/pdb6qh6/pdb
PDB entry 6qh6 coloured by chain, front view.
PDB entry 6qh6 coloured by chain, side view.
PDB entry 6qh6 coloured by chain, top view.
Source organisms:
Primary publication:
Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
Wrobel AG, Kadlecova Z, Kamenicky J, Yang JC, Herrmann T, Kelly BT, McCoy AJ, Evans PR, Martin S, Müller S, Salomon S, Sroubek F, Neuhaus D, Höning S, Owen DJ
OpenAccess logo Dev Cell 50 494-508.e11 (2019)
PMID: 31430451

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-134531 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 69.64 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 592 amino acids
Theoretical weight: 67.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 446 amino acids
Theoretical weight: 51.12 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
AP-2 complex subunit mu Chain: N
Molecule details ›
Chain: N
Length: 446 amino acids
Theoretical weight: 51.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
TGN38 CARGO PEPTIDE Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 809 Da
Source organism: Homo sapiens
Expression system: Not provided
T-cell surface glycoprotein CD4 Chain: Q
Molecule details ›
Chain: Q
Length: 10 amino acids
Theoretical weight: 1.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P41212
Unit cell:
a: 180.5Å b: 180.5Å c: 210.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.259 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Enzymatic Noncovalent Synthesis.
He et al. (2020)
3 more

2 mentions without citation

Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
Wrobel et al. (2019)
1 more