› 6pq7

X-ray diffraction
3Å resolution

Structure of the iMango-III fluorescent aptamer at room temperature.

Released:
DOI: 10.2210/pdb6pq7/pdb
PDB entry 6pq7 coloured by chain, front view.
PDB entry 6pq7 coloured by chain, side view.
PDB entry 6pq7 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Co-crystal structure of the iMango-III fluorescent RNA aptamer using an X-ray free-electron laser.
Trachman RJ, Stagno JR, Conrad C, Jones CP, Fischer P, Meents A, Wang YX, Ferré-D'Amaré AR
Acta Crystallogr F Struct Biol Commun 75 547-551 (2019)
PMID: 31397326

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197118 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (37-MER) Chain: C
Molecule details ›
Chain: C
Length: 37 nucleotides
Theoretical weight: 12.05 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand OXV 1 x OXV
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE MFX
Spacegroup: I4122
Unit cell:
a: 53.56Å b: 53.56Å c: 194.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.269 0.304
Expression system: Not provided

Quick links

Citations

2 citation in other articles

Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET.
Jeng et al. (2021)
1 more

3 mentions without citation

Thoughts on how to think (and talk) about RNA structure.
Vicens et al. (2022)
2 more