6oy5

X-ray diffraction
3.1Å resolution

X-ray crystal structure of a bacterial reiterative transcription complex of pyrG promoter at 3 min

Released:
Source organism: Thermus thermophilus
Entry authors: Shin Y, Murakami KS

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.06 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9Z9H6 (Residues: 1-315; Coverage: 100%)
Gene name: rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1119 amino acids
Theoretical weight: 125.44 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q8RQE9 (Residues: 1-1119; Coverage: 100%)
Gene names: TTHA1813, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1524 amino acids
Theoretical weight: 171 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q8RQE8 (Residues: 1-1524; Coverage: 100%)
Gene names: TTHA1812, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 99 amino acids
Theoretical weight: 11.53 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: A0A1J1EUF1 (Residues: 1-99; Coverage: 100%)
Gene names: TTHN1_00070, TTMY_2030, TthAA229_16300, TthHC11_16190, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 423 amino acids
Theoretical weight: 48.6 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q72L95 (Residues: 1-423; Coverage: 100%)
Gene names: TT_C0164, rpoD, sigA
Sequence domains:
DNA (5'-D(P*CP*CP*GP*CP*AP*TP*CP*AP*GP*AP*GP*CP*CP*CP*AP*AP*AP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 22 nucleotides
Theoretical weight: 6.66 KDa
DNA (5'-D(*AP*TP*AP*AP*TP*GP*GP*GP*AP*GP*CP*CP*TP*CP*TP*GP*AP*TP*GP*CP*AP*G)-3') Chain: H
Molecule details ›
Chain: H
Length: 27 nucleotides
Theoretical weight: 8.41 KDa
RNA (5'-D(*(GTP))-R(P*G)-3') Chain: I
Molecule details ›
Chain: I
Length: 2 nucleotides
Theoretical weight: 805 Da

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 186.106Å b: 102.417Å c: 297.237Å
α: 90° β: 99° γ: 90°
R-values:
R R work R free
0.208 0.207 0.259