› 6otd

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-coupled histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.37 KDa
Source organism: Anaeromyxobacter sp. Fw109-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7HD43 (Residues: 2-161; Coverage: 42%)
Gene names: Anae109_2438, gchK
Sequence domains: Protoglobin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand MPD 1 x MPD
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6122
Unit cell:
a: 69.44Å b: 69.44Å c: 113.364Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.182 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Applications of the Whole-Cell System in the Efficient Biosynthesis of Heme.
Su et al. (2023)
4 other articles

17 mentions without citation

Quadruplex Ligands in Cancer Therapy.
Sanchez-Martin et al. (2021)
16 more