PDBe 6oi4

X-ray diffraction
1.76Å resolution

RPN13 (19-132)-RPN2 (940-952) pY950-Ub complex

Released:
Source organism: Homo sapiens
Entry authors: Hemmis CW, Hill CP

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasomal ubiquitin receptor ADRM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16186 (Residues: 20-132; Coverage: 28%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13 ubiquitin receptor
Ubiquitin Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
26S proteasome non-ATPase regulatory subunit 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 amino acids
Theoretical weight: 1.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99460 (Residues: 940-952; Coverage: 1%)
Gene name: PSMD1

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P31
Unit cell:
a: 99.373Å b: 99.373Å c: 41.705Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli