6oeq

Electron Microscopy
4.3Å resolution

Cryo-EM structure of mouse RAG1/2 12RSS-PRC/23RSS-NFC complex (DNA1)

Released:
Source organism: Mus musculus
Primary publication:
Cutting antiparallel DNA strands in a single active site.
Nat. Struct. Mol. Biol. (2020)
PMID: 32015552
Related structures: EMD-20034

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
V(D)J recombination-activating protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1040 amino acids
Theoretical weight: 119.39 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P15919 (Residues: 1-1040; Coverage: 100%)
Gene name: Rag1
Sequence domains:
V(D)J recombination-activating protein 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 527 amino acids
Theoretical weight: 59.14 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P21784 (Residues: 1-527; Coverage: 100%)
Gene names: Rag-2, Rag2
Sequence domains:
DNA (57-MER) Chain: G
Molecule details ›
Chain: G
Length: 61 nucleotides
Theoretical weight: 18.81 KDa
DNA (57-MER) Chain: J
Molecule details ›
Chain: J
Length: 61 nucleotides
Theoretical weight: 18.79 KDa
DNA (46-MER) Chain: F
Molecule details ›
Chain: F
Length: 50 nucleotides
Theoretical weight: 15.53 KDa
DNA (46-MER) Chain: I
Molecule details ›
Chain: I
Length: 50 nucleotides
Theoretical weight: 15.28 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-20034
Expression system: Homo sapiens