6o9z

Electron Microscopy
3.03Å resolution

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to eukaryotic translation initiation factor 2 from Homo sapiens

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Translation initiation factor eIF-2B subunit epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 721 amino acids
Theoretical weight: 80.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains:
Translation initiation factor eIF-2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 368 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 2-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit delta Chains: E, F
Molecule details ›
Chains: E, F
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit gamma Chains: I, J
Molecule details ›
Chains: I, J
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Eukaryotic translation initiation factor 2 subunit 1 Chains: L, M
Molecule details ›
Chains: L, M
Length: 323 amino acids
Theoretical weight: 37.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05198 (Residues: 2-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-0664
Expression system: Escherichia coli