6o85

Electron Microscopy
3.03Å resolution

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to eukaryotic translation initiation factor 2 from Homo sapiens

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 13-mer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Translation initiation factor eIF-2B subunit epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains:
Translation initiation factor eIF-2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 368 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 2-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit delta Chains: E, F
Molecule details ›
Chains: E, F
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF-2B subunit gamma Chains: I, J
Molecule details ›
Chains: I, J
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Eukaryotic translation initiation factor 2 subunit 1 Chain: L
Molecule details ›
Chain: L
Length: 314 amino acids
Theoretical weight: 36.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05198 (Residues: 2-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
Eukaryotic translation initiation factor 2 subunit 3 Chain: S
Molecule details ›
Chain: S
Length: 472 amino acids
Theoretical weight: 51.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:
Eukaryotic translation initiation factor 2 subunit beta Chain: d
Molecule details ›
Chain: d
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-0651
Expression system: Escherichia coli