6mu4

X-ray diffraction
1.62Å resolution

Bst DNA polymerase I FANA/DNA binary complex

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 577 amino acids
Theoretical weight: 65.9 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1C9K5 (Residues: 4-580; Coverage: 100%)
Gene name: polA
Sequence domains: DNA polymerase family A
DNA (5'-D(P*GP*CP*GP*AP*TP*CP*AP*CP*GP*T)-3') Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
Source organism: synthetic construct
Expression system: Not provided
FANA (5'-D(P*(UF2)P*(A5L)P*(CFL)P*(GFL)P*(UF2)P*(GFL)P*(A5L)P*(UF2)P*(CFL)P*(GFL)P*(CFL))-3') Chain: T
Molecule details ›
Chain: T
Length: 13 nucleotides
Theoretical weight: 4.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
4 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 87.835Å b: 93.898Å c: 105.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.21
Expression systems:
  • Escherichia coli
  • Not provided