PDBe 6mol

X-ray diffraction
3.16Å resolution

Monoextended DARPin M_R12 complex with EpoR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Monoextended DARPin R12 (M_R12) Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 48.22 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Erythropoietin receptor Chains: B, C
Molecule details ›
Chains: B, C
Length: 229 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P19235 (Residues: 32-249; Coverage: 45%)
Gene name: EPOR
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P63
Unit cell:
a: 168.109Å b: 168.109Å c: 78.029Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.219 0.26
Expression systems:
  • Escherichia coli
  • Trichoplusia ni