› 6moj

X-ray diffraction
2.43Å resolution

Dimeric DARPin A_angle_R5 complex with EpoR

Released:
DOI: 10.2210/pdb6moj/pdb
PDB entry 6moj coloured by chain, front view.
PDB entry 6moj coloured by chain, side view.
PDB entry 6moj coloured by chain, top view.
Source organisms:
Primary publication:
Topological control of cytokine receptor signaling induces differential effects in hematopoiesis.
Mohan K, Ueda G, Kim AR, Jude KM, Fallas JA, Guo Y, Hafer M, Miao Y, Saxton RA, Piehler J, Sankaran VG, Baker D, Garcia KC
Science 364 (2019)
PMID: 31123111

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dimeric DARPin ACR5 (A_angle_R5) Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 24.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Erythropoietin receptor Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P19235 (Residues: 32-249; Coverage: 45%)
Gene name: EPOR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand TAR 3 x TAR
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I4122
Unit cell:
a: 130.418Å b: 130.418Å c: 293.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.25
Expression systems:
  • Escherichia coli
  • Trichoplusia ni

Quick links

Citations

12 review citations

Recent advances in de novo protein design: Principles, methods, and applications.
Pan et al. (2021)
11 more

63 mentions without citation

Antibody and B cell responses to SARS-CoV-2 infection and vaccination.
Röltgen et al. (2021)
62 more