6mnm

X-ray diffraction
3.1Å resolution

6256 TCR bound to I-Ab Padi4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class II histocompatibility antigen, A-B alpha chain Chain: C
Molecule details ›
Chain: C
Length: 180 amino acids
Theoretical weight: 20.31 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14434 (Residues: 27-205; Coverage: 77%)
Gene name: H2-Aa
Sequence domains:
H-2 class II histocompatibility antigen, A beta chain; Protein-arginine deiminase type-4 Chain: D
Molecule details ›
Chain: D
Length: 217 amino acids
Theoretical weight: 25.03 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14483 (Residues: 21-218; Coverage: 77%)
  • Canonical: Q9Z183 (Residues: 93-105; Coverage: 2%)
Gene names: H2-Ab1, H2-iabeta, Pad4, Padi4, Pdi4
Sequence domains:
6256 TCR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
6256 TCR beta chain Chain: B
Molecule details ›
Chain: B
Length: 239 amino acids
Theoretical weight: 26.76 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: C2
Unit cell:
a: 254.351Å b: 73.771Å c: 63.142Å
α: 90° β: 90.52° γ: 90°
R-values:
R R work R free
0.223 0.22 0.264
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)