6mdn

Electron Microscopy
4.4Å resolution

The 20S supercomplex engaging the SNAP-25 N-terminus (class 2)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 11-mer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-fusing ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 768 amino acids
Theoretical weight: 85.51 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 1-723; Coverage: 97%)
Gene name: NSF
Sequence domains:
Synaptosomal-associated protein 25 Chain: H
Molecule details ›
Chain: H
Length: 207 amino acids
Theoretical weight: 23.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 1-204; Coverage: 99%)
  • Best match: P60881-2 (Residues: 1-204)
Gene names: Snap, Snap25
Sequence domains: SNAP-25 family
Syntaxin-1A Chain: I
Molecule details ›
Chain: I
Length: 256 amino acids
Theoretical weight: 29.63 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 1-256; Coverage: 89%)
Gene names: Sap, Stx1a
Sequence domains: Syntaxin
Vesicle-associated membrane protein 2 Chain: J
Molecule details ›
Chain: J
Length: 117 amino acids
Theoretical weight: 12.98 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 1-72; Coverage: 62%)
Gene names: Syb2, Vamp2
Alpha-soluble NSF attachment protein Chains: K, L
Molecule details ›
Chains: K, L
Length: 313 amino acids
Theoretical weight: 35.6 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54921 (Residues: 1-278; Coverage: 94%)
Gene names: Napa, Snap, Snapa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-9101
Expression system: Escherichia coli