PDBe 6m92

X-ray diffraction
2.35Å resolution

Monophosphorylated pSer33 b-Catenin peptide, b-TrCP/Skp1, NRX-2663 ternary complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-box/WD repeat-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 49.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y297 (Residues: 175-605; Coverage: 71%)
Gene names: BTRC, BTRCP, FBW1A, FBXW1A
Sequence domains:
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 144 amino acids
Theoretical weight: 16.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 2-163; Coverage: 88%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Catenin beta-1 Chain: C
Molecule details ›
Chain: C
Length: 33 amino acids
Theoretical weight: 3.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P35222 (Residues: 17-48; Coverage: 4%)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P31
Unit cell:
a: 82.59Å b: 82.59Å c: 111.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.221
Expression systems:
  • Escherichia coli
  • Not provided