6k4u

X-ray diffraction
3.2Å resolution

Crystal structure of xCas9 in complex with sgRNA and DNA (TGA PAM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1368 amino acids
Theoretical weight: 158.54 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
target DNA Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.57 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
non-target DNA Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
sgRNA Chain: A
Molecule details ›
Chain: A
Length: 83 nucleotides
Theoretical weight: 26.79 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 178.005Å b: 70.314Å c: 190.077Å
α: 90° β: 110.34° γ: 90°
R-values:
R R work R free
0.223 0.22 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided