6k3a

X-ray diffraction
2.3Å resolution

Crystal structure of human PCNA in complex with DNMT1 PIP box motif.

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 264 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
DNA (cytosine-5)-methyltransferase 1 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 20 amino acids
Theoretical weight: 2.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P26358 (Residues: 161-180; Coverage: 1%)
Gene names: AIM, CXXC9, DNMT, DNMT1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 141.54Å b: 81.598Å c: 68.105Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.22 0.217 0.264
Expression systems:
  • Escherichia coli
  • Not provided