6j5b

X-ray diffraction
2.7Å resolution

Structural basis for the target DNA recognition and binding by the MYB domain of phosphate starvation response regulator 1

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein PHOSPHATE STARVATION RESPONSE 1 Chains: A, C, D, F, H, J
Molecule details ›
Chains: A, C, D, F, H, J
Length: 60 amino acids
Theoretical weight: 6.91 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94CL7 (Residues: 224-283; Coverage: 15%)
Gene names: At4g28610, PHR1, T5F17.60
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
DNA (5'-D(*GP*GP*TP*AP*CP*AP*GP*TP*AP*TP*AP*TP*AP*CP*CP*AP*TP*AP*AP*A)-3') Chains: B, E, I
Molecule details ›
Chains: B, E, I
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*AP*TP*GP*GP*TP*AP*TP*AP*TP*AP*CP*TP*GP*TP*AP*CP*C)-3') Chains: G, K, U
Molecule details ›
Chains: G, K, U
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 53.581Å b: 53.581Å c: 98.884Å
α: 91.47° β: 91.47° γ: 94.79°
R-values:
R R work R free
0.22 0.218 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided