PDBe 6hks

X-ray diffraction
2.19Å resolution

Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 114 amino acids
Theoretical weight: 12.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26045 (Residues: 489-597; Coverage: 12%)
Gene names: PTPH1, PTPN3
Sequence domains: PDZ domain
Protein E6 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 11 amino acids
Theoretical weight: 1.39 KDa
Source organism: Human papillomavirus type 16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03126 (Residues: 148-158; Coverage: 7%)
Gene name: E6

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 46.62Å b: 77.43Å c: 130.03Å
α: 90° β: 90.14° γ: 90°
R-values:
R R work R free
0.197 0.194 0.247
Expression system: Escherichia coli BL21(DE3)