X-ray diffraction
1.2Å resolution

Structure of SPF45 UHM bound to HIV-1 Rev ULM


Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 45 Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96I25 (Residues: 301-401; Coverage: 25%)
Gene names: RBM17, SPF45
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Protein Rev Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.4 KDa
Source organism: Human immunodeficiency virus 1
Expression system: synthetic construct
  • Canonical: P04618 (Residues: 41-49; Coverage: 8%)
Gene name: rev

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 48.21Å b: 63.68Å c: 67.63Å
α: 90° β: 90° γ: 90°
R R work R free
0.15 0.149 0.175
Expression systems:
  • Escherichia coli
  • synthetic construct