PDBe 6hep

X-ray diffraction
1.86Å resolution

Crystal structure of human 14-3-3 beta in complex with CFTR R-domain peptide pS753-pS768


Function and Biology Details

Reaction catalysed:
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P31946 (Residues: 1-232; Coverage: 94%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
Cystic fibrosis transmembrane conductance regulator Chains: E, F
Molecule details ›
Chains: E, F
Length: 28 amino acids
Theoretical weight: 3.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P13569 (Residues: 747-774; Coverage: 2%)
Gene names: ABCC7, CFTR

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 70.619Å b: 111.599Å c: 130.75Å
α: 90° β: 90° γ: 90°
R R work R free
0.204 0.201 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided