X-ray diffraction
1.88Å resolution

Crystal structure of E. coli tyrRS in complex with 5'-O-(N-L-tyrosyl)sulfamoyl-cytidine


Function and Biology Details

Reaction catalysed:
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 424 amino acids
Theoretical weight: 47.59 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0AGJ9 (Residues: 1-424; Coverage: 100%)
Gene names: JW1629, b1637, tyrS
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 81.611Å b: 65.049Å c: 90.686Å
α: 90° β: 101.35° γ: 90°
R R work R free
0.194 0.193 0.224
Expression system: Escherichia coli BL21(DE3)