PDBe 6gnn

X-ray diffraction
3.79Å resolution

Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31946 (Residues: 1-239; Coverage: 97%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
Exoenzyme T Chain: C
Molecule details ›
Chain: C
Length: 246 amino acids
Theoretical weight: 26.72 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I788 (Residues: 235-457; Coverage: 49%)
Gene names: PA0044, exoT
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 115.378Å b: 60.301Å c: 81.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.285 0.385
Expression system: Escherichia coli