PDBe 6gnj

X-ray diffraction
3.24Å resolution

Exoenzyme S from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, trimeric crystal form in complex with STO1101

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31946 (Residues: 1-234; Coverage: 95%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
Exoenzyme S Chain: C
Molecule details ›
Chain: C
Length: 244 amino acids
Theoretical weight: 26.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93SQ1 (Residues: 233-453; Coverage: 49%)
Gene name: exoS
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 160.398Å b: 59.357Å c: 120.641Å
α: 90° β: 125.84° γ: 90°
R-values:
R R work R free
0.177 0.173 0.221
Expression system: Escherichia coli