PDBe 6gn0

X-ray diffraction
3.24Å resolution

Exoenzyme S from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 28.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31946 (Residues: 1-239; Coverage: 97%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
Exoenzyme S Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 244 amino acids
Theoretical weight: 26.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93SQ1 (Residues: 234-453; Coverage: 49%)
Gene name: exoS
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21212
Unit cell:
a: 165.2Å b: 168.76Å c: 82.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.294
Expression system: Escherichia coli