6gfw

Electron Microscopy
3.7Å resolution

Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme initial transcribing complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma-54 factor Chain: M
Molecule details ›
Chain: M
Length: 497 amino acids
Theoretical weight: 54.77 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P06223 (Residues: 1-477; Coverage: 100%)
Gene names: ntrA, rpoN
Sequence domains:
nifH promoter template DNA Chain: F
Molecule details ›
Chain: F
Length: 63 nucleotides
Theoretical weight: 19.4 KDa
NifH promoter non-template DNA Chain: G
Molecule details ›
Chain: G
Length: 63 nucleotides
Theoretical weight: 19.49 KDa
de novo synthesized RNA Chain: R
Molecule details ›
Chain: R
Length: 4 nucleotides
Theoretical weight: 1.3 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4397
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'