PDBe 6gfn

X-ray diffraction
2.86Å resolution

METTL16 MTase domain

Released:
Source organism: Homo sapiens
Primary publication:
Methylation of Structured RNA by the m
Mol. Cell (2018)
PMID: 30197299

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5-(N(7)-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA] = S-adenosyl-L-homocysteine + a 5-(N(7)-methyl 5-triphosphoguanosine)-N(6),2'-O-dimethyladenosine-[mRNA]
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA N6-adenosine-methyltransferase METTL16 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86W50 (Residues: 1-291; Coverage: 52%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4122
Unit cell:
a: 92.36Å b: 92.36Å c: 180.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.222
Expression system: Escherichia coli BL21(DE3)