6g85

X-ray diffraction
1.53Å resolution

Structure of Cdc14 bound to CBK1 PxL motif

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-156898 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tyrosine-protein phosphatase CDC14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 42.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00684 (Residues: 1-374; Coverage: 68%)
Gene names: CDC14, OAF3, YFR028C
Sequence domains:
Serine/threonine-protein kinase CBK1 Chain: C
Molecule details ›
Chain: C
Length: 16 amino acids
Theoretical weight: 1.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P53894 (Residues: 82-97; Coverage: 2%)
Gene names: CBK1, N1727, YNL161W
Serine/threonine-protein kinase CBK1 Chain: D
Molecule details ›
Chain: D
Length: 17 amino acids
Theoretical weight: 1.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P53894 (Residues: 81-97; Coverage: 2%)
Gene names: CBK1, N1727, YNL161W

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 76.154Å b: 97.408Å c: 129.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.209
Expression systems:
  • Escherichia coli BL21
  • Not provided