PDBe 6ezn

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 54.12 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P41543 (Residues: 1-476; Coverage: 100%)
Gene names: J1404, NLT1, OST1, YJL002C
Sequence domains: Ribophorin I
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 Chain: B
Molecule details ›
Chain: B
Length: 130 amino acids
Theoretical weight: 14.71 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P46964 (Residues: 1-130; Coverage: 100%)
Gene names: OST2, YOR103C, YOR3211C
Sequence domains: DAD family
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 350 amino acids
Theoretical weight: 39.52 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P48439 (Residues: 1-350; Coverage: 100%)
Gene names: OST3, YOR085W, YOR3124W
Sequence domains: OST3 / OST6 family, transporter family
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 Chain: D
Molecule details ›
Chain: D
Length: 36 amino acids
Theoretical weight: 3.99 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q99380 (Residues: 1-36; Coverage: 100%)
Gene names: OST4, YDL232W
Sequence domains: Oligosaccaryltransferase
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 Chain: E
Molecule details ›
Chain: E
Length: 86 amino acids
Theoretical weight: 9.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q92316 (Residues: 1-86; Coverage: 100%)
Gene names: OST5, YGL226BC, YGL226C-A
Sequence domains: Oligosaccharyltransferase subunit 5
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 Chain: F
Molecule details ›
Chain: F
Length: 718 amino acids
Theoretical weight: 81.6 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P39007 (Residues: 1-718; Coverage: 100%)
Gene names: STT3, YGL022W
Sequence domains: Oligosaccharyl transferase STT3 subunit
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 Chain: G
Molecule details ›
Chain: G
Length: 430 amino acids
Theoretical weight: 49.44 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33767 (Residues: 1-430; Coverage: 100%)
Gene names: WBP1, YEL002C
Sequence domains: Oligosaccharyltransferase 48 kDa subunit beta
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 Chain: H
Molecule details ›
Chain: H
Length: 286 amino acids
Theoretical weight: 31.68 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02795 (Residues: 1-286; Coverage: 100%)
Gene names: SWP1, YM9375.19, YMR149W
Sequence domains: Oligosaccharyltransferase subunit Ribophorin II

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-4161