6evk

X-ray diffraction
2.9Å resolution

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter and cap analogue m7GTP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 738 amino acids
Theoretical weight: 85.49 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 809 amino acids
Theoretical weight: 91.03 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*GP*GP*G)-3') Chain: V
Molecule details ›
Chain: V
Length: 16 nucleotides
Theoretical weight: 5.25 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 269.34Å b: 148.7Å c: 88.51Å
α: 90° β: 98.17° γ: 90°
R-values:
R R work R free
0.236 0.234 0.273
Expression system: Trichoplusia ni