6eht

X-ray diffraction
3.2Å resolution

Modulation of PCNA sliding surface by p15PAF suggests a suppressive mechanism for cisplatin-induced DNA lesion bypass by pol eta holoenzyme

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Proliferating cell nuclear antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P12004 (Residues: 1-254; Coverage: 97%)
Gene name: PCNA
Sequence domains:
Proliferating cell nuclear antigen Chain: C
Molecule details ›
Chain: C
Length: 256 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli O103:H2 str. 12009
UniProt:
  • Canonical: P12004 (Residues: 1-255; Coverage: 98%)
Gene name: PCNA
Sequence domains:
PCNA-associated factor Chains: D, E
Molecule details ›
Chains: D, E
Length: 20 amino acids
Theoretical weight: 2.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15004 (Residues: 52-71; Coverage: 18%)
Gene names: KIAA0101, L5, NS5ATP9, PAF, PCLAF
DNA (5'-D(P*AP*TP*AP*CP*GP*AP*TP*GP*GP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 10 nucleotides
Theoretical weight: 3.11 KDa
DNA (5'-D(P*CP*CP*CP*AP*TP*CP*GP*TP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 10 nucleotides
Theoretical weight: 2.98 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 75.993Å b: 42.3Å c: 141.827Å
α: 90° β: 102.7° γ: 90°
R-values:
R R work R free
0.264 0.261 0.327
Expression systems:
  • Escherichia coli K-12
  • Escherichia coli O103:H2 str. 12009
  • Not provided